猪瘟病毒通过网格蛋白介导的内吞途径入侵ST细胞

doi:10.11843/j.issn.0366-6964.2017.01.017

Abstract

Abstract:

Cell entry of virus is an indispensable process in virus life cycle. However, the mechanism of classical swine fever virus (CSFV) cell entry is still undiscovered. Here, CSFV entry into ST cells by clathrin-mediated endocytosis was tested. Chemical inhibitor chlorpromazine and Dynasore and shRNA were used to disturb the clathrin-mediated endocytosis. The cell entry efficiency of CSFV was greatly decline when the function of clathrin and dynamin-2 were blocked. By using NH₄Cl and down-regulation of Rab5 and Rab7 gene, CSFV infection was inhibited. These results showed that the CSFV can entry into susceptible cells by using clathrin-mediated endocytosis pathway and the early endosome and late endosome were used for CSFV infection. This research provides new information to understand the CSFV infection.

CLC Number:

S852.651

LIANG Wu-long，FANG Jia，LIN Zhi，ZHENG Min-ping，BAO Chang-lei，WANG Tao，ZHANG Yan-ming. Classical Swine Fever Virus Entry into ST Cells by Clathrin-mediated Endocytosis Pathway[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(1): 140-149.

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Classical Swine Fever Virus Entry into ST Cells by Clathrin-mediated Endocytosis Pathway

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